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Reproductive fluids, added to the culture media, contribute to minimizing phenotypical differences between in vitro-derived and artificial insemination-derived piglets

Published online by Cambridge University Press:  06 January 2022

Evelyne París-Oller Open the ORCID record for Evelyne París-Oller [Opens in a new window] ,
Cristina Soriano-Úbeda ,
Ramsés Belda-Pérez ,
Lucía Sarriás-Gil ,
Jordana S. Lopes ,
Analuce Canha-Gouveia ,
Joaquín Gadea ,
Luis Alberto Vieira ,
Francisco Alberto García-Vázquez  and
Raquel Romar
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Evelyne París-Oller
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain
Cristina Soriano-Úbeda
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain
Ramsés Belda-Pérez
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain
Lucía Sarriás-Gil
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain
Jordana S. Lopes
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain Biomedical Research Institute of Murcia (IMIB), Murcia, Spain
Analuce Canha-Gouveia
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain Biomedical Research Institute of Murcia (IMIB), Murcia, Spain
Joaquín Gadea
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain Biomedical Research Institute of Murcia (IMIB), Murcia, Spain
Luis Alberto Vieira
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain
Francisco Alberto García-Vázquez
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain Biomedical Research Institute of Murcia (IMIB), Murcia, Spain
Raquel Romar
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain Biomedical Research Institute of Murcia (IMIB), Murcia, Spain
Sebastian Cánovas
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain Biomedical Research Institute of Murcia (IMIB), Murcia, Spain
Pilar Coy*
Affiliation:
Department of Physiology, University of Murcia, Campus of Excellence Mare Nostrum, Murcia, Spain Biomedical Research Institute of Murcia (IMIB), Murcia, Spain
*
Address for correspondence: Pilar Coy, Department of Physiology, University of Murcia, Murcia, Spain. Email: pcoy@um.es
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Abstract

The addition of reproductive fluids (RF) to the culture media has shown benefits in different embryonic traits but its long-term effects on the offspring phenotype are still unknown. We aimed to describe such effects in pigs. Blood samples and growth parameters were collected from piglets derived from in vitro-produced embryos (IVP) with or without RF added in the culture media versus those artificially inseminated (AI), from day 0 to month 6 of life. An oral glucose tolerance test was performed on day 45 of life. We show here the first comparative data of the growth of animals produced through different assisted reproductive techniques, demonstrating differences between groups. Overall, there was a tendency to have a larger size at birth and faster growth in animals derived from in vitro fertilization and embryo culture versus AI, although this trend was diminished by the addition of RFs to the culture media. Similarly, small differences in hematological indices and glucose tolerance between animals derived from AI and those derived from IVP, with a sex-dependent effect, tended to fade in the presence of RF. The addition of RF to the culture media could contribute to minimizing the phenotypical differences between the in vitro-derived and AI offspring, particularly in males.

Keywords

Assisted reproductive technologiesin vitro embryo productionreproductive fluidsgrowthhematological parametersglucose tolerance
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 13 , Issue 5 , October 2022 , pp. 593 - 605
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Copyright
© The Author(s), 2022. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease

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