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Quantitative assessment of degradation, cytocompatibility, and in vivo bone regeneration of silicon-incorporated magnesium phosphate bioceramics

Published online by Cambridge University Press:  30 December 2019

Vinod Kumar ,
Kaushik Sarkar ,
Karuppasamy Bavya Devi ,
Debaki Ghosh ,
Samit Kumar Nandi  and
Mangal Roy
Vinod Kumar
Affiliation:
Department of Veterinary Surgery & Radiology, West Bengal University of Animal & Fishery Sciences, Kolkata 700037, India
Kaushik Sarkar
Affiliation:
Department of Metallurgical and Materials Engineering, Indian Institute of Technology-Kharagpur, Kharagpur 721302, India
Karuppasamy Bavya Devi
Affiliation:
Department of Metallurgical and Materials Engineering, Indian Institute of Technology-Kharagpur, Kharagpur 721302, India
Debaki Ghosh
Affiliation:
Department of Veterinary Surgery & Radiology, West Bengal University of Animal & Fishery Sciences, Kolkata 700037, India
Samit Kumar Nandi*
Affiliation:
Department of Veterinary Surgery & Radiology, West Bengal University of Animal & Fishery Sciences, Kolkata 700037, India
Mangal Roy*
Affiliation:
Department of Metallurgical and Materials Engineering, Indian Institute of Technology-Kharagpur, Kharagpur 721302, India
*
a)Address all correspondence to these authors. e-mail: samitnandi1967@gmail.com
b)e-mail: mroy@metal.iitkgp.ac.in
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Abstract

The effects of silicon incorporation on the in vitro and in vivo properties of magnesium phosphate (MgP) bioceramics were studied. Samples were prepared by conventional solid state synthesis method. Scanning electron microscopy and micro-computed tomography (µ-CT) analysis showed that Si doping reduces degradability of MgP. In vitro studies have shown that MG63 cells can attach and proliferate on MgP samples. Live/dead imaging showed that MgP–0.5Si sample had highest cell proliferation, which was also quantitatively confirmed by alamar blue assay. In vivo biocompatibility of MgP ceramics was assessed after implantation in rabbit model. Detailed µ-CT analysis showed new bone tissue formation around the implant after 30 and 90 days. MgP–0.5Si ceramics had 84% bone regeneration compared with 56% for pure MgP ceramics, as confirmed by oxytetracycline labeling. Our finding suggests that Si doping can alter physicochemical properties of MgP ceramics and promotes osseointegration, which can be a useful choice for bone tissue engineering.

Keywords

magnesium phosphatebioceramicdegradationin vitroin vivomicro-CT
Type
Article
Information
Journal of Materials Research , Volume 34 , Issue 24 , 30 December 2019 , pp. 4024 - 4036
Copyright
Copyright © Materials Research Society 2019 

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Footnotes

c)

These authors contributed equally to this work.

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